Investigation of Rusty Guard Crabs on Pocillopora in Costa Rica

Investigation of Rusty Guard Crabs on Pocillopora in Costa Rica

Rusty Guard Crabs sheltered in a Pocillopora Coral

One of our recent interns, Andre Wax, completed and presented a research study on a topic of his choosing! Read about Andre’s work below.

Trapezia Bidentata, also known as the Rusty Guard Crab, are the local species of guard crabs found on Pocillopora corals near Quepos – Manuel Antonio region of the Eastern Tropical Pacific in Costa Rica. They are a species of smaller guard crab, maxing out at around 2.5 cm in length, and provide protection against smaller corallivores. The Pocillopora corals that they inhabit are species of branching corals, which provide shelter for the guard crabs. T. Bidentata will also stimulate the coral to release mucus by massaging the coral with their legs and collect the mucus the coral excretes and will consume this as their food source.

 This study aims to discover if there is a relationship between the health of Pocillopora corals in the Quepos – Manuel Antonio area and the number of Trapezia Bidentata crabs found living in the coral. A null and alternative hypothesis were generated, where the null stated that there would be no relationship found while the alternative hypothesis stated that the number of T. Bidentata crabs found on the corals would depend on the health of the coral. If the alternative was true, it could be found that on healthier corals there are more guard crabs. Furthermore, larger corals likely have more crabs due to there being more space available.  

Initial findings

The depth, length, number of T. Bidentata present, and coral health were recorded. Coral health was recorded using a scale from zero to four, representing dead and healthy respectively. Initially, depth was compared with the number of crabs and the length of the coral, and no correlation was found (Figure 1.1 and 1.2).

Depth in feet against the Number of Crabs found at each depth
Depth against the Length of Coral found at each depth

 When coral length and number of crabs were compared with each other a strong correlation was found. The trendline generated from this data allowed for the data to be analyzed for the corals that had more crabs than expected (data points above the trendline) and corals that had less crabs than expected (data points below the trendline).  Samples that had more crabs than expected had health ratings of 3.5 or 4 most frequently, with a few data points with a health rating of 3 and only one data point that was below a health rating of 3 (see figure 1.3). 

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Number of crabs against the length of the coral in centimetres

 When coral length and number of crabs were compared with each other a strong correlation was found. The trendline generated from this data allowed for the data to be analyzed for the corals that had more crabs than expected (data points above the trendline) and corals that had less crabs than expected (data points below the trendline).  Samples that had more crabs than expected had health ratings of 3.5 or 4 most frequently, with a few data points with a health rating of 3 and only one data point that was below a health rating of 3 (see figure 1.3). Corals with less crabs than expected were generally corals that we unhealthy.  When the frequency of finding different numbers of crabs on the corals was compared, it was revealed that as the corals get healthier, crabs would be found in higher numbers more often than compared to the corals that are unhealthy, and high numbers of crabs are only found on corals that are the healthiest (see figure 2.1).

Frequency of number of crabs with a health rating between 0 and 0.5

Finally, based analysis of the data, the corals were separated into two categories, healthy corals (3-4 health rating), and unhealthy corals (0-2.5). Box and whisker plots were utilized to remove outliers and ensure normal distribution before conducting a two-variable t-test assuming unequal variances (see figure 3.1 and 3.2). This test was selected due to the possibility of unequal standard deviation among data sets and due to the large sample size. Using α = 0.05, the two hypotheses were H0 (T. Bidentata number is different when comparing health of Pocillopora corals): p ≥ 0.05 and HA (T. Bidentata number is different when comparing health of Pocillopora corals): p<0.05. When conducted, a p-value of  4.175*10-8 was obtained. 4.175*10-8 < α = 0.05 so the null hypothesis is rejected.  



Distribution of the number of T.Bidentata Crabs
Distribution of the number of T.Bidentata crabs excluding outliers

Analysis of the data reveals that T. Bidentata were found in larger quantities on the healthier corals. The coral length vs. crab number data reveals that very few unhealthy corals can have more crabs than expected, and this is supported by the t-test which resulted in an incredibly low p-value, suggesting a very strong significant difference between the number of T. Bidentata on healthy vs. unhealthy corals.

One possible reason for this could be that unhealthy corals are unable to sustain higher numbers of T. Bidentata. Since dead coral could not be able to produce mucus for the guard crabs to feed on, it would not be possible for guard crabs to survive on the coral. 

 

The feeding strategy of crabs could also better prepare the immune system of the corals for future stressors. When corals release mucus, it is part of their immune response to stressors in their environment.

Rusty Guard Crabs on Outplanted Coral

There are several reasons that this could be occurring. Since guard crabs feed on the mucus from the corals, unhealthy corals would supply guard crabs with less food so a lower number of guard crabs would be found. Another possibility is that a higher number of crabs would allow the coral to be more resistant to stressors in the environment. Corals with higher numbers of crabs would have more crabs to protect against corallivores. This would keep the corals healthier because predator attacks would be less successful in consuming the coral. The feeding strategy of Trapezia crabs could also keep corals healthier. When they feed, they stimulate the immune response of corals causing them to release mucus. Keeping the immune response stimulated could keep the coral more prepared for stressors that would cause this immune response. 

Guard crabs such as T. Bidentata can be used in the future as an effective indicator species. Due to the strong relationship the number of guard crabs found on Pocillopora corals can be used to quantify the health of the corals. The benefits that this can provide is another way to determine coral health besides visual examination. 

When fragmenting corals, it is important to ensure that guard crabs can remain on the corals. Guard crabs need to be on corals to survive, so if they are removed from corals during the fragmenting process, it is essential to reattach them to a fragment and attempt to keep the crabs on the fragments through the out-planting process. When fragmenting, cutting slowly allows the crabs time to move out of the way of the blade.



Guard Crabs found during the fragmenting process

Trapezia Bidentata crabs play an important role in the reefs in the Quepos – Manuel Antonio area. With invasive species such as the Crown of Thorns seastar entering Costa Rica, the protection guard crabs provide is going to be essential in ensuring the survival of the reefs. Further research on this subject could include examining the coral health and guard crabs in a controlled setting, examining the size and number of crabs found depending on the shape and structure of corals, and comparing the health of corals and the number of guard crabs in coral nurseries. 



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Behind the Scenes with our Marine Biology Interns

Behind the Scenes with our Marine Biology Interns

Here at Marine Conservation Costa Rica, we have a number of opportunities available for those looking to make a difference to Marine wildlife. Hear about the experiences of two of our previous interns, Andre and Mary-Grace below.

What is the sea life like?

Andre

It’s a lot of fish. The visibility is not that good, so it is hard to see other animals. A lot of damsels farm and eat algae and surgeonfish. Occasionally you’ll see octopus, sharks and turtles but it is much rarer than the other common reef fish.

Mary-Grace

At Quepos it is very interesting. The diving is amazing and there are more surges, which makes it harder to dive. I feel like this makes you a more experienced diver because it is a challenge. In Caño the water is much more still and calm.

How would you describe the variety of sea life? Do they seem healthy and diverse?

Andre

The sea life is very diverse with a lot of different types of fish!

Mary-Grace

The animals here are quite diverse, we have seen a bunch of different fish like the butterfly fish and sergeant majors, sea slugs, and some crabs on the nursery corals.

How would you describe the Coral you see?

Andre

There is not a lot of reef building coral so generally, it’s pretty sparse. We see some of the branching coral and some of the bouldering coral which are big and look like boulders.

Mary-Grace

We have two types of corals: the branching and boulder corals. The branching coral grows like a tree, and the boulder coral grows on the ground.

Do you encounter Coral bleaching more than healthy reefs?

Andre

You definitely see a lot of both. At Caño Island there is definitely a lot of bleached coral but here in Quepos there is less bleached coral. A lot of the coral is fairly young, but you see a fair share of both.

Mary-Grace

Here in Quepos, we have some coral bleaching and coral disease, but we can’t tell which it is without doing research on it. We definitely do see coral bleaching, more at Caño Island than here in Quepos.

As a diver do you practice a marine conservation lifestyle? If so, what does this look like for you?

Andre

Yes, mostly using reusable plastics and bottles. I would also say making conscious choices of where you are throwing things away, so pollutants don’t end up in the ocean.

Mary-Grace

Personally, I try to be conservative about what I use such as avoiding single-use plastics. If I do I try to reuse them as much as possible. I will keep my trash collected until I am near a trash can to avoid it getting to the ocean.

Describe the process of new Coral colonies being planted.

Andre

I haven’t done much with the coral because I am with the marine conservation side but most of the coral are very small.

Mary-Grace

In the restoration program we find coral of opportunity which is coral floating around, and not breaking off coral from a healthy reef. From there we pull it out and fragment it (cut it a little) to help the growth accelerate. We let them grow in the nursery for a few months before planting them back onto the larger coral structure.

Describe the process of new Coral colonies being planted.

Andre

I haven’t done much with the coral because I am with the marine conservation side but most of the coral are very small.

Mary-Grace

In the restoration program we find coral of opportunity which is coral floating around, and not breaking off coral from a healthy reef. From there we pull it out and fragment it (cut it a little) to help the growth accelerate. We let them grow in the nursery for a few months before planting them back onto the larger coral structure.

What inspired you to be a diver?

Andre

I have always really liked the ocean since I was a kid so it is my plan to enter marine biology. Diving is very helpful for researching the ocean and its’ biology and is a big interest of mine.

Mary-Grace

For me, I have wanted to dive since I was young. I did a discovery course and realized I was too scared. With it being on my mind this past year, I finally decided to face my fears and did it. I quickly realized there was nothing to be scared of at all.

What inspired you to take action with Marine conservation?

Andre

I care about conservation in general, but diving is a part of it. Being out in nature and the environment is important because we as humans are a part of nature. It’s important because without it we would die too.

Mary-Grace

When I was little, my sister showed me the movie Blue Ocean and it showed me that our oceans need help with restoration. Corals are extremely important for the health of our oceans and that is why I want to take action and help build a healthy ocean.

Has anything surprised you?

Andre

The visibility was a little surprising at first. I’m always surprised when I see a lot of garbage at the dive sites. A lot of plastics are seen.

Mary-Grace

Yes, when diving in Quepos I was expecting a coral reef with lots of animals and abundant life. Instead, I saw a lot of emptiness which I wasn’t expecting. I was expecting more Great Barrier Reef style but that just isn’t the reality for most coral reefs.

During your time here, what was your most memorable experience?

Andre

Last week we saw a turtle that was swimming around us and as I swam back to my group it started chasing me out of its territory, so that was pretty cool.

Mary-Grace

My most memorable experience was last week when I found my first nudibranch. They’re barely an inch big and very hard to find. I was super excited to find my first one.

Like what you hear and Interested in taking part yourself? Just click the button below to read more about the Internships available to you!

My Experience as an Environmental Education Intern

My Experience as an Environmental Education Intern

My Environmental Education Experience 

 

social media intern

One of our recent interns, Joe came and joined us for a month, assisting with our environmental education program. This is about his experience with us. 

In my time working with Marine Conservation Costa Rica (MCCR) I have experienced growth and bonding, and learned exceedingly. Working in the Environmental Education internship, I have helped to organize lessons, learn new teaching skills, and about the importance and intricacies of conservation. Not to mention, I have hugely improved on my Spanish.

 I had the unique opportunity to go into a primary school and teach in Spanish about marine conservation. While this sounded daunting to me at first, the experience was invaluable. Teaching gave me confidence and a better understanding of how to help people learn in a productive and educational way. It was great because not only was I teaching others, but I was also learning too. I went from knowing limited Spanish to far more competent very quickly and felt comfortable speaking, answering and understanding Spanish. I was also helped every step of the way by the fantastic team at MCCR who allowed me to feel optimistic in the face of a big task. 

First things first…

First, my teaching at the school consisted of prep work. I created a powerpoint in English then in Spanish about my chosen topic for the children. After creating the powerpoint and speaker notes, I went over them a few times with the team to make sure I felt comfortable with the pronunciation and was ready to speak it to the kids. Jointly, I worked to prepare an activity for the children, to create a more fun and interactive setting. Finally, I was ready.

I went in and was greeted by the staff and set up my presentation. Once I was up there, all of my nerves suddenly vanished and my head cleared, I was prepared and excited now. I thoroughly enjoyed doing my presentation and was able to answer and understand the kids’ questions, which was neat. I proceeded smoothly to the activity in which we helped the kids paint their own sharks and then play a game using the sharks they had made. 

An enjoyable experience

The children seemed to really enjoy the whole day which made it even more of a fulfilling experience. I also had the fantastic opportunity to help teach about coral conservation and fragmentation to an older group of teenagers at the event. This involved speaking to multiple different groups about the complexities of coral reefs and helping with hands-on activities on fragmenting coral to help replant the reefs. This not only granted further confidence but really helped to teach me about the work people do for conservation, the importance of it and the intricacies of the marine fauna we are trying to protect. On top of all of this, there are other activities all interns and supporters can participate in.

MCCR often organizes group activities that provide unforgettable experiences, such as a trip to the mangroves to see crocodiles. The fantastic team, other interns and general positive and friendly people around it all means super fun group trips to beautiful waterfalls, beaches or other fun activities such as football are a commonplace to why I always felt involved and like my time was being spent well, making the most, of the beautiful country of Costa Rica and Quepos. 

Want to get involved ?

With all of the projects we work hard on throughout the year, we are always looking for help. You can get involved with one of our internship or volunteer programs.

Apply Now

Content creator and Social Media intern

Content creator and Social Media intern

Get Involved

 

social media intern
outplanting corals

We need your help to spread our message of awareness

We have been super busy this year already with both the nurseries, and some exciting new projects coming soon. We are still small and growing fast and we need your help to keep this momentum going. We are looking for two interns. One to help us with content creation for our media channels, and one for managing the social media channels that we have. Both positions are for 3-6 months depending on the person, possibly longer. Our hope is that we can then find some enthusiastic eco warriors to help spread awareness and our message to our expanding network of supporters. And find some new ones in the process. Outlines for the two positions are found below. We look forward to hearing from you!

What do you get in return?

You get to work with us and help spread awareness for the plight of the oceans!

Not just that, obviously! We are offering either a full marine conservation internship in exchange or if you are a looking to become a professional diver we can discuss the ECO Divemaster option with you as well. We have options for housing and homestays available as well.

If you are not a diver then we can work with that as well. We can make that happen as it is important to us that you have a good understanding of what we do. In order to do that, you are better off underwater!

Social Media Intern

We are looking for an enthusiastic social media intern to join us. You will be responsible for creating social media campaigns and the day-to-day management of MCCRs social media social media posts and channels. You need a passion for social media and marine conservation.

The successful intern will be an excellent communicator, a versatile creative writer, and a team player. You will be able to manage our channels through scheduling software, analyze posts and campaigns, and interact with our network of enthusiastic eco warriors.

 

coral nursery costa rica

Requisites

We ask that you have experience in the management of social media channels and scheduling software. Also, that you understand branding and production of effective and engaging social media posts.

You can work closely with our content creator

Knowledge of Spanish is a bonus.

Scuba diving experience is desirable.

To apply

  • Please submit a current Resume (CV) with references
  • Please submit an example of how you would plan and manage a campaign on a new artifical reef project.

 

Note: Can be offered as part of a university degree work experience/Thesis project if desired. Please outline in your application.

 

Content Creator Intern

suAs Content Creator at Marine Conservation Costa Rica, you’ll be in charge of creating online content like blog posts, newsletters, social media posts both visual and written. This content will help us reach our eco warriors. It’s up to you to provide them with valuable information about our projects and more. 

You will need to be able to create both written and visual content so you will need to be able to take photos, videos around the dive center and on our projects.  Access to a Gopro is perfect as this can also be used underwater. If you do not have any experience underwater, we have Divemasters and instructors around who very often can provide underwater footage which you can then utilize.

We hope that you will be able to assist us with creating more education materials for our programs and projects.

 

coral nursery costa rica

Requisites

You have the ability to write for multiple channels 
You have excellent writing and editing skills.
You have a sense of branding and know how to keep a consistent tone of voice in your writing and media
You have experience working with a CMS like WordPress.
You can create basic visuals and videos
You know how to optimize your writing for SEO.
You have a passion for the underwater environment.

You can work closely with our social media intern.

Knowledge of Spanish is a bonus

Scuba diving experience is desireable

To apply

  • Please submit a current Resume (CV) with references
  • Please submit some examples of your writing and photo/video content

 

Note: Can be offered as part of a university degree work experience/Thesis project if desired. Please outline in your application.

 

Apply Now

Coral diary update – a busy start to November

Coral diary update – a busy start to November

Coral Coral Coral! We have been really busy working in our coral nurseries these last couple of weeks. Kat and our new intern Anni have cleaned and measured all our current coral fragments; we can report some great growth since the last data collection a month ago! 

coral fragmenting

Tool fundraiser update

We are thrilled that our “Tool Fundraiser” has allowed us to purchase a Gryphon AquaSaw! Thank you so much to our generous donors that made this purchase possible!
The Aquasaw is a diamond blade bandsaw, and is ideal for precision fragmenting of hard corals. This is going to totally change the way we can process corals to go out to the nurseries.

Working on our coral fragmenting in Costa Rica

Tuesday was for fragmenting!

On Tuesday we collected corals from wild donor colonies and brought them back to the Marina for processing. We used the new saw to “micro-fragment” the corals into smaller pieces to grow in the nurseries. The saw makes it much easier to cut the fragments into the perfect size and causes less stress to the corals. The coral fragments were then attached to concrete discs on which they will grow in our ocean nurseries for the next 6 month to a year. Cutting the corals into small fragments stimulates growth, we will also be monitoring and cleaning to give them and even better chance of survival. 

Marine conservation costa rica intern Anni

Coral planting here we come

On Wednesday, we are excited to announce that we took over 100 fragments of 3 coral species and planted them in our nurseries!!
If you would like to help fund our coral reef restoration work, our tool fundraiser is still going! We have several more tools that we need including an underwater drill, that will use for attaching the coral colonies grown in our nurseries back onto our local reef.

Pura Vida,

Kat